Forensic DNA Profiling Protocols
Patrick J Lincoln (Editor), Jim Thomson (Editor)
In Forensic DNA Profiling Protocols, leading forensic experts from around the world describe in detail their time-proven methods for identity testing through DNA analysis. Their state-of-the-art collection of easily reproducible methods includes all of the major techniques of DNA analysis currently used in forensic identity testing. The book covers PCR-based test systems, the now widely used STR typing systems (complete with details of both manual and automated detection systems), repeat unit mapping (MVR), direct-phase minisequencing, capillary electrophoresis, and direct blotting. Also fully discussed are the recovery of DNA from a wide range of sample types (blood, semen, skeletal remains, and saliva), species testing, sex determination, and mitochondrial DNA testing. Forensic DNA Profiling Protocols captures and makes practical for all laboratories the whole array of new technologies and techniques that have revolutionized forensic science. The readily reproducible methods elucidated here represent the state-of-the-art for those scientists who want to introduce, establish, and perfect DNA identification techniques in their laboratories
Laboratory manuals
1 online resource (321 pages)
9781280830792, 9786610830794, 9781592592043, 9780896034433, 1280830794, 6610830797, 159259204X, 0896034437
1097154537
Available in another form:
Recovery of High-Molecular-Weight DNA from Blood and Forensic Specimens
Recovery of DNA for PCR Amplification from Blood and Forensic Samples Using a Chelating Resin
PCR Analysis of DNA from Fresh and Decomposed Bodies and Skeletal Remains in Medicolegal Death Investigations
PCR Analysis from Cigaret Butts, Postage Stamps, Envelope Sealing Flaps, and Other Saliva-Stained Material
Quantification of DNA By Slot-Blot Analysis
Rapid Assessment of PCR Product Quality and Quantity by Capillary Electrophoresis
Southern Blotting of Genomic DNA for DNA Profiling
Preparation and Use of Alkaline-Phosphatase-Conjugated Oligonucleotide Probes for Single-Locus and Multilocus VNTR Analysis
Preparation and Use of 32P-Labeled Single-Locus VNTR Probes in Identity Testing
Statistical Methods Employed in Evaluation of Single-Locus Probe Results in Criminal Identity Cases
Interpretation and Statistical Evaluation of Multilocus DNA Fingerprints in Paternity and Relationship Testing
Overview of PCR-Based Systems in Identity Testing
An Introduction to PCR Primer Design and Optimization of Amplification Reactions
Analysis of Amplified Fragment-Length Polymorphisms (VNTR/STR Loci) for Human Identity Testing
Amplification of Short Tandem Repeat Loci Using PCR
Manual Electrophoretic Methods for Genotyping Amplified STR Loci
17 Genotyping STR Loci Using an Automated DNA Sequencer
Separation of PCR Fragments by Means of Direct Blotting Electrophoresis
19 Amplification and Sequencing of Mitochondrial DNA in Forensic Casework
Minisatellite Variant Repeat Unit Mapping Using PCR (MVR-PCR)
Sex Determination by PCR Analysis of the X-Y Amelogenin Gene
Species Determination by Analysis of the Cytochrome b Gene
Use of the AmpliType PM + HLA DQA1 PCR Amplification and Typing Kits for Identity Testing
The Use of Capillary Electrophoresis in Genotyping STR Loci
Solid-Phase Minisequencing as a Tool to Detect DNA Polymorphism
English