In Vitro Biological Systems : Methods in Toxicology, Vol. 1
Methods in Toxicology, Volume 1: In Vitro Biological Systems, Part A provides basic techniques employed by widely recognized scientists to prepare and maintain the biological components of in vitro model systems. The book discusses the in vitro models of neural and neuromuscular systems; ocular system; respiratory system; cardiovascular system; and gastrointestinal system. The text also describes liver slices; liver hepatocytes; other liver cell systems; proximal tubule fragments; kidney cell culture; reproductive and developmental systems; immune system; and skin. Pharmacologists, toxicologis
eBook, English, 2014
Elsevier Science, Saint Louis, 2014
Methods in toxicology, v. 1
1 online resource (595 pages).
9781483218601, 1483218600
1040673150
Front Cover; Methods in Toxicology; Copyright Page ; Table of Contents; Contributors; Preface; Introduction; References; SECTION I: NEURAL AND NEUROMUSCULAR SYSTEMS; CHAPTER 1. HIPPOCAMPAL SLICES; INTRODUCTION; MATERIALS; PROCEDURES; COMMENTS; REFERENCES; CHAPTER 2. OGANOTYPIC NEURAL CULTURES; INTRODUCTION; MATERIALS AND REAGENTS; PROCEDURES; COMMENTS; ACKNOWLEDGMENTS; REFERENCES; CHAPTER 3. REAGGREGATE CULTURES FOR NEUROTOXICOLOGICAL STUDIES; INTRODUCTION; MATERIALS AND REAGENTS; PROCEDURES; EVALUATION OF NEUROTOXICITY; EXPERIMENTAL APPROACHES; COMMENTS; REFERENCES. CHAPTER 4. CYTOTOXICITY IN MURINE NEOCORTICAL CELL CULTUREINTRODUCTION; CULTURE MEDIA AND SOLUTIONS; CULTURE PROCEDURES; EXPERIMENTAL PROTOCOLS; DISCUSSION; ACKNOWLEDGMENTS; REFERENCES; CHAPTER 5. CULTURES OF ADULT TRIGEMINAL GANGLION NEURONS; INTRODUCTION; MATERIALS AND REAGENTS; PROCEDURES; COMMENTS; ACKNOWLEGMENTS; REFERENCES; CHAPTER 6. ADRENOMEDULLARY CHROMAFFIN CELLS FOR TOXICOLOGICAL STUDIES; INTRODUCTION; MATERIALS AND REAGENTS; PROCEDURES; COMMENTS; ACKNOWLEDGMENTS; REFERENCES; CHAPTER 7. PC-12 CELLS; INTRODUCTION; MATERIALS AND REAGENTS; PROCEDURES; COMMENTS; ACKNOWLEDGMENTS. CHAPTER 10. ISOLATION AND CULTURE OF TYPE II ALVEOLAR EPITHELIAL CELLSINTRODUCTION; ISOLATION OF TYPE II CELLS; TYPE II CELL CULTURES; CONCLUSIONS; APPENDIX: ISOLATION OF TYPE II CELLS FROM RABBIT LUNG; ACKNOWLEDGMENTS; REFERENCES; CHAPTER 11. ISOLATION OF NONCILIATED BRONCHIOLAR (CLARA) EPITHELIAL CELL FROM MOUSE LUNG; INTRODUCTION; MATERIALS AND REAGENTS; PROCEDURE; COMMENTS; ACKNOWLEDGMENTS; REFERENCES; CHAPTER 12. RAT TRACHEAL EPITHELIAL CELL CULTURES FOR STUDIES IN TOXICOLOGY AND CARCINOGENICITY; INTRODUCTION; MATERIALS AND METHODS; SUMMARY AND CONCLUSION; REFERENCES. SECTION IV: CARDIOVASCULAR SYSTEMCHAPTER 13. PREPARATION OF PRIMARY CULTURES OF POSTNATAL RAT MYOCARDIAL CELLS FOR TOXICOLOGICAL STUDIES; INTRODUCTION; MATERIALS AND REAGENTS; PROCEDURES; COMMENTS; ACKNOWLEDGMENTS; REFERENCES; CHAPTER 14. AORTIC ENDOTHELIAL AND SMOOTH MUSCLE CELL CULTURES; INTRODUCTION; MATERIALS AND REAGENTS; PROCEDURE; COMMENTS; ACKNOWLEDGMENTS; REFERENCES; CHAPTER 15. VESSEL CYLINDERS; INTRODUCTION; PROCEDURES, MATERIALS, AND REAGENTS; COMMENTS; REFERENCES; SECTION V: GASTROINTESTINAL SYSTEM; CHAPTER 16. GASTRIC MUCOSAL CELL CULTURE FOR TOXICOLOGICAL STUDY; INTRODUCTION
MATERIALS AND REAGENTS